Evaluation of Analytical Sensitivity of Sdf I based PCR and Sandwich ELISA for Salmonella Enteritidis detection and On-Farm prevalence in Punjab, Pakistan

Salmonella Enteritidis (SE) is a dominant serotype among non-typhoidal which renders poultry products unsafe for human consumption. Due to frequent reporting of egg associated outbreaks, broiler breeder flocks are understudied although farm environment present supporting conditions the growth SE. In this study, two rapid detection techniques SE were compared in terms analytical sensitivity and extent contamination was determined. Analytical as limit (LOD) evaluated quantitatively specific PCR based on amplification Sdf I gene commercially available sandwich ELISA antigen detection. triplicate experiments, tenfold serial dilutions prepared tested with each technique. Using pure cultures, found be 18.6 CFU/ml 2.77×105 respectively. (LOD, log 1.2) more sensitive than 5.4). Environmental swab samples (n = 260) collected from 22 hen houses representing 8 farms located around Lahore Sheikhupura districts Punjab province. From house litter, nests, feeders, drinkers, fans, pads, ceiling, walls walkways. Following selective enrichment, pooled subjected PCR. Results showed that 36.3 % (8/22) detected positive These findings suggest improvement biosecurity measures advocate implementation integrated Salmonellosis control programs minimize carcass contamination.